SOP On Cleaning and Disinfection of Microbiology Laboratory
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Objective
To lay down the procedure for Cleaning and Disinfection of microbiology laboratory.
Scope
This SOP is applicable for Cleaning and Disinfection of microbiology laboratory.
Responsibility
Microbiologist or above of Microbiology Laboratory.
Head – Microbiology section / Nominee
Abbreviations and Definitions
MLT : Microbial Limit Test
V/V : Volume by Volume
LAF : Laminar Air Flow
IPA : Isopropyl Alcohol
WFI : Water for injection
Microbiology Laboratory consists :
General Microbiology Laboratory
Microbiology entry air lock, Microbiology general corridor, Microbiology Sterilization area, Media preparation area, Washing area, Media decontamination area, Microbiology office, Incubator room & Glassware storage room.
Sterility testing area of Microbiology Laboratory
Change room 1, Change room 2, Change room 3, Change room 4, Sterility Testing room, Sterile Buffer Room, Return Buffer and Return Air Lock.
MLT Section of Microbiology Laboratory
MLT A/L & Change room, MLT room and Subculture room.
Clean and disinfect all areas of microbiology lab twice a day (Morning and evening).
General Microbiology Laboratory :
Using a lint free mopping duster remove dust from all surfaces of COB, doors, window glasses, walls, incubator surfaces, grills, external surfaces of equipment like ovens, autoclave, refrigerator, weighing balance, pH meter, water bath, Tables etc.
Sweep the floor to remove the dust using non-shedding lint-free dry mop.
Take two buckets in which add purified water in one bucket and add dilute disinfectant solution in another bucket.
Mop the surfaces with cloth that is wetted purified water after that mop with dilute disinfectant.
details of Microscope as per the annexure in SOP No.
Clean the drains, sinks and wash basins with purified water after flushing with disinfecting solution at frequency daily.
Clean and sanitize the media preparation table and washing area table with 70% IPA solution.
Empty the waste bins twice daily and clean the bins with water and disinfectant solution.
Remove the dust from the ceiling, shelves, ducts, lights, fixtures, racks etc., using vacuum cleaner monthly.
Wipe the floor with the floor cleaning solution.
Clean the S.S. Garment and shoe cabinet present in the primary change over area daily using a clean dry cloth.
Record the details of cleaning in cleaning and sanitization of microbiology lab.
Procedures for organizations and undertake microbiological tests
List of Disinfectant to be used:
- Lysol – 2.5% v/v
- Domex-2.5% v/v
- Savlon-2.5% v/v
Sequence of rotation:
On weekly rotation; one disinfectant for a week.
Frequency of cleaning:
Twice a day.
Sterility testing area of Laboratory:
Clean and disinfect the Garment cabinet and the used garment cabinet present in the Change room-1 with a lint free cloth wetted with 70 % IPA.
Clean the floor, glass surfaces, LAF hood with WFI and wipe with sterile lint free mop pads.
Sprinkle the disinfectant on the floor and spread with sterile lint free mop pads.
Allow for the contact period of 10 minutes.
Wipe out the excess disinfectant with the sterile lint free mop pads using a long handled SS mop.
Follow the above steps in a sequence from testing room to change rooms.
Sanitize the dedicated slippers with the same disinfectant as used for the floor daily by dipping them in disinfectant and allowing the contact period of 10 minutes. After completions of the contact period dip in WFI, spray 70% IPA and allow drying.
Clean the autoclave door, Garment cabinet, pass box daily with 70% IPA solution.
Clean all the surfaces, trolleys and SS furniture daily with the disinfectant.
Change the disinfectants every week and use in rotation as below.
List of Disinfectant to be used:
- Triple 100 1% v/v
- Combatan DS 1% v/v
- Protasan DS 1% v/v
Sequence of rotation:
On weekly rotation; one disinfectant for a weekly
Frequency of cleaning
Twice a day.
MLT Section of Laboratory :
Clean and disinfect the used garment box and the cross over bench daily with 70 % I.P.A.
Clean the floor, glass surfaces, LAF hood with WFI and wipe with sterile lint free mop pads.
Sprinkle the disinfectant on the floor and spread with sterile lint free mop pads.
Allow for the contact period of 10 minutes.
Wipe out the excess disinfectant with the sterile lint free mop pads using a long handled SS mop.
Sanitize the dedicated slippers with the same disinfectant as used for the floor daily by dipping them in disinfectant and allowing the contact period of 10 min. After completion of the contact period dip in WFI, spray 70% IPA and allow to dry.
Change the disinfectants every week and use in rotation as below.
List of Disinfectant to be used:
- Triple 100 1% v/v
- Combatan DS 1% v/v
- Protasan DS 1% v/v
- Use of Cidex for spraying in testing areas is weekly .
Sequence of rotation:
On weekly rotation; one disinfectant for a week.
Frequency of cleaning:
Twice a day.
Record the details of cleaning and disinfection
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