Glassware for Microbiology
PURPOSE
To lay down the procedure for Cleaning Procedure of Glassware in Microbiology Lab in Microbiology Lab.
SCOPE:
This SOP is applicable to provide the procedure for Cleaning Procedure of Glassware in Microbiology Lab.
Procedure for cleaning of microbiological Laboratory
RESPONSIBILITY:
Microbiologist Person shall be responsible for:
To follow this procedure during Cleaning Procedure of Glassware in Microbiology Lab & maintain the record.
Head QC/Designee:
o review new or revised SOP.
To maintain the controlled copies of SOPs at required location
Head QA/Designee
To approve new or revised SOP.
To ensure implementation of system as per defined SOP
Plant Head
To approve new or revised SOP
To ensure implementation of system as per defined SOP.
PROCEDURE
SAFETY/PRECAUTION/EHS
Carefully handle the glassware to prevent any breakage of glassware which may harm to the person handling the glassware.
Always wear nose mask and gloves while washing the glassware.
Do not use the glassware which is partially broken, it may injured you.
Use sufficient volume of raw water to completely remove the traces of surfactant.
Procedure For Glassware washing
Collect all the glassware to be cleaned in washing area.
Prepare sufficient amount of 0.2% v/v Teepol solution into purified water and record the preparation detail..
Dip the glassware into teepol solution and use soft nylon brush to remove any traces of media on glass surface.
Rinse the glassware with raw water until the complete removal of soap solution from the glassware.
Now finally rinse the glassware with purified water and keep it on the washing bench in inverted position so the water shall be remove from the glassware.
Now load all the washed glassware into the drying oven and operate the oven as per SOP.
Keep all washed glassware into the drying oven at least for one hour to completely dry the glassware.
After drying use the glassware for different activities in micro lab.
Procedure for Footwear Cleaning
Prepare sufficient amount of 2% v/v Teepol solution into purified water and record the preparation detail..
Footwear shall be cleaned by dipping it in 2% Teepol solution followed by raw water rinsing until complete removal of soap solution and finally rinse with purified water and record the Cleaning detail.
Frequency: Weekly ±2 working days or whenever required.
Procedure for Garment washing
Garments for aseptic area (Sterility) and other cleaned room i.e. MLT, Culture Testing Disinfectant Preparation shall be washed every time after its use. While aprons and caps used in unclassified area shall be washed twice in a week in laundry.
Garments used in Sterility shall be cleaned separately.
Ten Garment at a time /or as per feasibility.
Botties shall be cleaned separately.
Adequate Surfactant quantity should be used to clean the garments.
Garment used during sub culturing shall be cleaned separately i.e. Garments should not be cleaned with other garment.
