SOP On Cleaning and Disinfection of microbiology Laboratory

A microbiology laboratory is a laboratory devoted to the culturing, examination, and identification of microorganisms including bacteria, fungi, yeasts, etc. Clinical – Managing the infections on a regular basis Epidemiological – Deep knowledge of an infective microbe present in a patient is helpful in investigating its source and mode of transmission. As well as causing a familiar range of diseases in animals and plants and problems in food spoilage and deterioration of other materials, microbes are also our “invisible allies”. The purpose of this manual is to provide teachers and technicians with good techniques in practical microbiology to ensure that investigations proceed safely and achieve the required educational aims successfully.

Practices and Safety Rules for  Microbiology Laboratory Wash your hands with disinfectants when you arrive at the lab and again before you leave. Wear laboratory coats in the lab. At the start and end of each laboratory session, students should clean their assigned bench-top area with a disinfectant solution provided. Report any breakage of equipment to the instructor. Discard all cultures and used glassware into the container labeled Contaminated. Plastic or other disposable items should be discarded separately from glassware in containers to be sterilized. Before beginning your laboratory exercise, wash off the bench top with the disinfectant provided. When exercises are completed, wash off the bench top again. Always wash your hands with soap and water before leaving the laboratory. Before leaving the laboratory, see that all the equipment’s are in the proper location and gas and water turned off. Use them to clearly label your cultures. If you should spill or drop a culture or if any type of accident occurs, call the instructor immediately. Place a paper towel over any spill and pour disinfectant over the towel. Let the disinfectant stand for 15 minutes and then clean the spill with fresh paper towels. Remember to discard the paper towels in the proper receptacle and wash your hands carefully. Disinfect work areas before and after use with 70% alcohol or fresh 10% bleach. Laboratory equipment and work surfaces should be decontaminated with an appropriate disinfectant on a routine basis and especially after spills, splashes or other contamination. Cultures are not to be removed from the laboratory unless the instructor gives permission. i.e. , flood areas with disinfectant if cultures are spilled, wash hands after contact and notify your instructor at once. Also, make sure you have all the materials needed for the exercise at hand before you commence the experiment.

Objective

To lay down the procedure for Cleaning and Disinfection of microbiology laboratory.

Scope

This SOP is applicable for Cleaning and Disinfection of microbiology laboratory.

Responsibility

Microbiologist or above of Microbiology Laboratory.

Head – Microbiology section / Nominee

Abbreviations and Definitions

MLT         : Microbial Limit Test

V/V           : Volume by Volume

LAF         : Laminar Air Flow

IPA           : Isopropyl Alcohol

WFI         : Water for injection

Microbiology Laboratory consists :

General Microbiology Laboratory

Microbiology entry air lock, Microbiology general corridor, Microbiology Sterilization area, Media preparation area, Washing area, Media decontamination area, Microbiology office, Incubator room & Glassware storage room.

Sterility testing area of Microbiology Laboratory

Change room 1, Change room 2, Change room 3, Change room 4, Sterility Testing room, Sterile Buffer Room, Return Buffer and Return Air Lock.

MLT Section of Microbiology Laboratory

MLT A/L & Change room, MLT room and Subculture room.

Clean and disinfect all areas of microbiology lab twice a day (Morning and evening).

General Microbiology Laboratory :

Using a lint free mopping duster remove dust from all surfaces of COB, doors, window glasses, walls, incubator surfaces, grills, external surfaces of equipment like ovens, autoclave, refrigerator, weighing balance, pH meter, water bath, Tables etc.

Sweep the floor to remove the dust using non-shedding lint-free dry mop.

Take two buckets in which add purified water in one bucket and add dilute disinfectant solution in another bucket.

Mop the surfaces with cloth that is wetted purified water after that mop with dilute disinfectant.

details of Microscope as per the annexure in SOP No.

Clean the drains, sinks and wash basins with purified water  after flushing with disinfecting solution at frequency daily.

Clean and sanitize the media preparation table and washing area table with 70% IPA solution.

Empty the waste bins twice daily and clean the bins with water and disinfectant solution.

Remove the dust from the ceiling, shelves, ducts, lights, fixtures, racks etc., using     vacuum cleaner monthly.

Wipe the floor with the floor cleaning solution.

Clean the S.S. Garment and shoe cabinet present in the primary change over area   daily using a clean dry cloth.

Record the details of cleaning in cleaning and sanitization of microbiology lab.

Procedures for organizations and undertake microbiological tests

List of Disinfectant to be used:

• Lysol – 2.5% v/v
• Domex-2.5% v/v
• Savlon-2.5% v/v

Sequence of rotation:

On weekly rotation; one disinfectant for a week.

Frequency of cleaning:

Twice a day.

Sterility testing area of Laboratory:

Clean and disinfect the Garment cabinet and the used garment cabinet present in the Change room-1 with a lint free cloth wetted with 70 % IPA.

Clean the floor, glass surfaces, LAF hood with WFI and wipe with sterile lint free mop pads.

Sprinkle the disinfectant on the floor and spread with sterile lint free mop pads.

Allow for the contact period of 10 minutes.

Wipe out the excess disinfectant with the sterile lint free mop pads using a long   handled SS mop.

Follow the above steps in a sequence from testing room to change rooms.

Sanitize the dedicated slippers with the same disinfectant as used for the floor daily by dipping them in disinfectant and allowing the contact period of 10 minutes. After completions of the contact period dip in WFI, spray 70% IPA and allow drying.

Clean the autoclave door, Garment cabinet, pass box daily with 70% IPA solution.

Clean all the surfaces, trolleys and SS furniture daily with the disinfectant.

Change the disinfectants every week and use in rotation as below.

List of Disinfectant to be used:

• Triple 100 1% v/v
• Combatan DS 1% v/v
• Protasan DS 1% v/v

Sequence of rotation:

On weekly rotation; one disinfectant for a weekly

Frequency of cleaning

Twice a day.

MLT Section of Laboratory :

Clean and disinfect the used garment box and the cross over bench daily with 70 % I.P.A.

Clean the floor, glass surfaces, LAF hood with WFI and wipe with sterile lint free mop pads.

Sprinkle the disinfectant on the floor and spread with sterile lint free mop pads.

Allow for the contact period of 10 minutes.

Wipe out the excess disinfectant with the sterile lint free mop pads using a long   handled SS mop.

Sanitize the dedicated slippers with the same disinfectant as used for the floor daily by dipping them in disinfectant and allowing the contact period of 10 min. After completion of the contact period dip in WFI, spray 70% IPA and allow to dry.

Change the disinfectants every week and use in rotation as below.

List of Disinfectant to be used:

• Triple 100 1% v/v
• Combatan DS 1% v/v
• Protasan DS 1% v/v
• Use of Cidex for spraying in testing areas is weekly .

Sequence of rotation:

On weekly rotation; one disinfectant for a week.

Frequency of cleaning:

Twice a day.

Record the details of cleaning and disinfection

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