Bacteriological Incubator

SOP of Bacteriological Incubator

The Bacteriological Incubator is utilized for the storage and growth of bacterial cultures at a temperature of 37°C. These incubators, also known as heated incubators, have a temperature range that remains above ambient temperature up to 60°C. They are equipped with a digital temperature controller and an air circulation fan, which ensures the uniform distribution of temperature throughout the chamber.

Every unit is equipped with a digital PID temperature controller to ensure high-temperature accuracy and uniformity. This controller displays both the set value (SV) and the process value (PV). It also includes a temperature low/high alarm that alerts the operator in case of any variation in the set temperature. Heat is generated using resistance wire heaters or beaded heaters, and a motorized blower or axial fan is installed to evenly distribute the temperature across the chamber.

Bacteriological incubators are constructed with durability in mind. The external cabinet is made of a thick gauge GI sheet, which is powder-coated and painted to enhance its corrosion resistance. A GMP model with a complete stainless steel construction if required. The inner chamber is made of thick gauge 304-grade stainless steel sheets. The gap between the inner and external chambers is filled with high-density glass wool, which minimizes heat loss and ensures energy efficiency.

Bacteriological Incubator

Temperature Control: Bacterial incubators are equipped with a microprocessor-based PID temperature controller and PT100 RTD sensor, allowing for precise temperature control within a range of ambient+10°C to 60°C. This controller displays both the Set Value (SV) and Process Value (PV), ensuring accurate monitoring of the temperature.

Air Circulation: Bacterial incubators feature both natural and forced air convection. To ensure uniform distribution of temperature throughout the chamber, a motorized blower is fitted at the back side of the chamber. The on/off switch provided at the panel allows for easy control of the blower.

Sturdy Construction: 37-degree bacteriological incubators are built with durability in mind. The external cabinet is made of a thick gauge GI sheet, which is powder-coated and painted to enhance its corrosion resistance. The inner chamber is constructed using thick gauge 304-grade stainless steel sheets. The gap between the inner and external chamber is filled with high-density glass wool, minimizing heat loss and making the unit energy efficient. Additionally, the door is equipped with an observation glass window, allowing for sample viewing without the need to open the door.

Safety Measures: Ensuring temperature safety is of utmost importance in bacteriological incubators. That’s why our units are equipped with a temperature thermostat and a low/high alarm system. This alarm alerts the operator in case of any variation in the set temperature, providing an added layer of safety.

To cater to these specific requirements, a range of customization options and accessories for bacterial incubators. These include digital timers, PLC-based HMI controllers, 21 CFR part 11 software, data loggers, and thermal printers, among others.

Technical Specification of Bacteriological Incubator
  • Temperature range
  • Temperature accuracy
  • Temperature controller
  • Temperature sensor
  • Inner chamber
  • External cabinet
  • Insulation
  • Door
  • Heaters
  • Shelves
  • Air circulation
  • Standard fittings
  • Power supply
  • PLC + HMI controller w/ USB interface
  • 21 CFR Part 11 Software
  • Data logger w/ USB interface
  • Thermal printer
  • Inner chamber LED light
Components of Bacteriological Incubator

Cabinet: The MOC of the cabinet body is stainless steel. The cabinet is coupled with an insulator, The Main Function of the cabinet is to prevent the heat from escaping and maintain the temperature.

Shelves: Shelves are used to provide space for placing the microbial culture.

Door: The Door is airtight because of the asbestos gasket that keeps heat trapped inside the incubator.

Thermostat: Thermostat are used to maintain the desired temperature. The thermostat contains a sensor that senses the temperature.

Control panel:  The control panel contains switches for all the parameters.

Supply humidity and gas: The gas (O2 and CO2) is maintained by the associated reservoirs. The humidity is maintained by the vaporizing of the water (if required).

SOP of Motility test by Hanging drop Method

About Pharmaceutical Guidanace

Ms. Abha Maurya is the Author and founder of pharmaceutical guidance, he is a pharmaceutical Professional from India having more than 18 years of rich experience in pharmaceutical field. During his career, he work in quality assurance department with multinational company’s i.e Zydus Cadila Ltd, Unichem Laboratories Ltd, Indoco remedies Ltd, Panacea Biotec Ltd, Nectar life Science Ltd. During his experience, he face may regulatory Audit i.e. USFDA, MHRA, ANVISA, MCC, TGA, EU –GMP, WHO –Geneva, ISO 9001-2008 and many ROW Regularities Audit i.e.Uganda,Kenya, Tanzania, Zimbabwe. He is currently leading a regulatory pharmaceutical company as a head Quality. You can join him by Email, Facebook, Google+, Twitter and YouTube

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