SOP of Media Decontamination and Disposal after used

SOP of Media Decontamination and Disposal after used

• Objective

To lay down the procedure for Decontamination and Disposal of Used Media

•  Scope

This SOP is applicable for Decontamination and Disposal of Used Media. (Pharmaceutical Company Name).

• Responsibility
  • Microbiologist or above of Microbiology Laboratory.
  • Head – Microbiology section / Nominee.

• Accountability
  • Head – Quality Control. / Nominee.

• Abbreviations and Definitions

SOP         : Standard operating procedure

No.            : Number

QC            : Quality Control

QA            : Quality Assurance

• Procedure
• Wear nose mask and disposable hand gloves during scrapping and decontamination activity of used media.
• For Solidified Media(Media Used for Settle plates exposure, Surface Monitoring test plates, Microbial limit test plates, Growth promotion test media, Sub-culture slants  etc.):
• Segregate and arrange the plates showing growth of colonies into biohazard bag.
• Scrap out the media from the plates having no growth with the help of spatula into another biohazard bag.
• Place the bags in the Vertical autoclave.
• For Liquefied Media: (Sterility Tubes/canisters, Positive Control tubes, culture tubes product enrichment tubes etc.)
• Place all the test tubes in the test tube stand and arrange them in the Vertical autoclave.
• Place the Disposable media plates/strips/media kits, used cotton plugs, gloves used
• for handling of microbial cultures, lyophilized culture glass pieces, sterility test vent filters & tubing etc in a biohazard bag and load in the vertical autoclave.
• Keep one or two deodorizing pearls (if available) in the decontamination load to prevent putrefied smell of the used media.
• Operate the vertical autoclave as per SOP No
• After sterilization, collect the media contained in tubes/ bottles and other decontaminated material into biohazard bag.
• Add 50mL of 20% Virosil solution or 2.5% Savlon to the bag containing decontaminated media and shake the bag for proper dispersion of the added disinfectant solution.
• Discard the contents of the bag into the washbasin/sink.
• Open the tap and allow the water to flow while discarding the contents.

 Forms and Records (Annexures)

• • Not applicable

• Distribution
  • Master copy        –       Quality Assurance
  • Controlled copies –       Quality Assurance, Production, Quality Control, Stores, Engineering   and Human Resource

• History:

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