SOP of Microscope
Objective of this SOP
To lay down the procedure for Operation, Cleaning and Maintenance of Microscope
Scope of this SOP
This SOP is applicable for Operation, Cleaning and Maintenance of Microscope in Microbiology Laboratory of (Pharmaceutical Company Name).
Responsibility of this SOP
Chemist or above of Microbiology Laboratory.
Head – Microbiology Section.
Accountability of this SOP
Head – Quality Control.
Abbreviations and Definitions used in this SOP
SOP : Standard operating procedure
No. : Number
QC : Quality Control
QA : Quality Assurance
Make: Leica, Model: Leica DME
Ensure that the microscope and its surrounding area is clean.
Plug the microscope power cord in to electrical out let.
Turn on the microscope by rotating the illumination control knob on the bottom left side of the instrument.
Set the intensity of light to the lowest setting using illumination control knob.
Fully open the aperture diaphragm of the condenser by rotating the ring to the extreme right.
Using the sub stage condenser-focusing knob, raise the condenser to the top of its excursion. Critical illumination only: If the condenser travel is excessive, limit the travel with the thumbscrew under the sub stage until the top lens of it is just below the stage surface (0.35mm)
Place the specimen slide on the stage.
Rotate the nosepiece to move the objective (40 X for dry mount and 10 X for wet mount or 100 X oil immersion) into working position.
Raise the stage by rotating the coarse adjustment knob to its positive stop. Using the fine adjustment knob, bring the specimen into sharp focus.
Adjust the eye tubes for inter pupillary distance and eye difference. The left eyepiece tube is focusable to compensate for refractive differences of the eyes.
To correctly set the eye tubes, focus on the specimen through the right eyepiece tube only. Use the fine adjustment knob while covering the left eyepiece or closing the left eye.
Next, focus the specimen through the left eyepiece by turning the eye tube. Cover the right eyepiece while doing this and be sure to focus with the left eye tube only, without using the focusing knob.
Remove an eyepiece and view the back aperture of the objective. Close the condenser aperture diaphragm and then, to obtain the full resolving power of the microscope, reopen until the diaphragm leaves just disappear from view. Replace the eyepiece. The aperture diaphragm can be adjusted to enhance contrast and/or increase the depth of focus.
When changing to higher power objectives, the positions of the aperture diaphragm must be reset. As magnification increases, the aperture diaphragm must be opened as required.
Cleaning and Maintenance:
Whenever lack of contrast, cloudiness or poor definition is encountered, Clean the lower magnification objectives and optical surfaces with a lint free cloth or lens tissue moistened (not wet) with methanol or commercially available glass cleaner.
Clean the front lens with a toothpick covered with a cotton tip wetted with methanol.
Avoid excessive use of solvent for cleaning.
Cover the microscope always with dust cover, whenever the microscope is not in use.
Wipe the bottom of Oil immersion lens of a fast absorbing tissue paper before and after using the Lens.
Use Xylene to clean the lens surfaces.
Record the cleaning details of Microscope as per the annexure in SOP No.
Forms and Records (Annexures)
Master copy – Quality Assurance
Controlled copies – Quality Assurance, Production, Quality Control, Stores, Engineering and Human Resource
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