DO’s & DON’Ts For Column care to avoid/Minimize incidences

DO’s & DON’Ts For Column care to avoid/Minimize incidences

1.0 New HPLC Columns(Except Silica Columns for Normal Phase)

DO’s

  • Before using the new column wash the column with water followed by an appropriate solvent mixture.
  • For Silica Column for washing use solvent as Given in the Column Certificate.

DON’Ts

  • Don’t saturate the new column directly with the mobile phase
  • Don’t use water for washing of new Silica column.

2.0 Existing column ( Before Analysis)

DO’s

  • Before analysis wash the column with water followed by the appropriate solvent mixture& then saturate the column with the mobile phase.

DON’Ts

  • Don’t saturate the Existing column directly with the mobile phase

3.0 Washing & Storage of HPLC column

DO’s

  • After analysis wash the column with the appropriate solvent mixture
  • For long storage (more than 1 month) store the HPLC column in a pure organic solvent

DON’Ts

  • Don’t store the column as it is without providing washing to the same.
  • For long storage (more than 1 month) don’t store the HPLC column in the solvent mixture. (Organic solvent: Buffer solution)

4.0 Dedicated column

DO’s

  • Use the Dedicated column which is mentioned in the HPLC column list

DON’Ts

  • Don’t Use other columns which is not mentioned in the HPLC column list.

5.0 End fitting

DO’s

  • After completion of analysis store the column with end fittings properly fitted.

DON’Ts

  • After completion of analysis don’t keep the column without end fitting.

About Pharmaceutical Guidanace

Ms. Abha Maurya is the Author and founder of pharmaceutical guidance, he is a pharmaceutical Professional from India having more than 18 years of rich experience in pharmaceutical field. During his career, he work in quality assurance department with multinational company’s i.e Zydus Cadila Ltd, Unichem Laboratories Ltd, Indoco remedies Ltd, Panacea Biotec Ltd, Nectar life Science Ltd. During his experience, he face may regulatory Audit i.e. USFDA, MHRA, ANVISA, MCC, TGA, EU –GMP, WHO –Geneva, ISO 9001-2008 and many ROW Regularities Audit i.e.Uganda,Kenya, Tanzania, Zimbabwe. He is currently leading a regulatory pharmaceutical company as a head Quality. You can join him by Email, Facebook, Google+, Twitter and YouTube

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