DO’s & DON’Ts For Column care to avoid/Minimize incidences

DO’s & DON’Ts For Column care to avoid/Minimize incidences

1.0 New HPLC Columns(Except Silica Columns for Normal Phase)

DO’s

  • Before using the new column wash the column with water followed by an appropriate solvent mixture.
  • For Silica Column for washing use solvent as Given in the Column Certificate.

DON’Ts

  • Don’t saturate the new column directly with the mobile phase
  • Don’t use water for washing of new Silica column.

2.0 Existing column ( Before Analysis)

DO’s

  • Before analysis wash the column with water followed by the appropriate solvent mixture& then saturate the column with the mobile phase.

DON’Ts

  • Don’t saturate the Existing column directly with the mobile phase

3.0 Washing & Storage of HPLC column

DO’s

  • After analysis wash the column with the appropriate solvent mixture
  • For long storage (more than 1 month) store the HPLC column in a pure organic solvent

DON’Ts

  • Don’t store the column as it is without providing washing to the same.
  • For long storage (more than 1 month) don’t store the HPLC column in the solvent mixture. (Organic solvent: Buffer solution)

4.0 Dedicated column

DO’s

  • Use the Dedicated column which is mentioned in the HPLC column list

DON’Ts

  • Don’t Use other columns which is not mentioned in the HPLC column list.

5.0 End fitting

DO’s

  • After completion of analysis store the column with end fittings properly fitted.

DON’Ts

  • After completion of analysis don’t keep the column without end fitting.