SOP on Fogging of Microbiology Testing Areas

• Objective:
  • To lay down the procedure for Fogging of Microbiology Testing Areas
• Scope:
  • This SOP is applicable for Fogging of Microbiology Testing Areas in Microbiology Laboratory.
• Responsibility:
  • Chemist or above of Microbiology Laboratory:
  • Head – Microbiology section.
• Accountability:
  • Head – Quality Control.
• Procedure:
  • Operation
    • Perform the Fogging of Microbiology once in a week at the end of working hours
    • Perform the fogging on below mentioned areas on different days Sterility testing area.
    • MLT, Subculture room and MLT section corridor and Change room.

3)   Sterilization area

• Make sure that the area is thoroughly cleaned before fogging.
• Close all the dampers.
• Switch off all the AHU.
• Enter into the area to be fogged as per the SOP along with the fogger (Micromist and Nanotech) filled with the required quantity of dilute disinfectant.
• Sterility testing area:
  • Switch off the interlocking of the sterility testing area.

• Enter into change room-4 as per the SOP for the area along with the fogger filled with dilute virosil.
• Keep the doors between change room-2 & 3 and change room 3 & 4 open.
• Place the fogger in one corner of change room-4.
• Adjust the motor angle at 45˚C and the timer to 0.5 liter.
• Connect the power cord of the fogger to the mains and switch on the fogger and quickly move out of the room into the sterility room and close the door.
• Wait in the sterility room for the fogging to complete.
• As soon as the fogging is complete, quickly transfer the fogger from change room-4 to Sterile Buffer room and place similarly.
• Adjust the timer to around 0.7 liter.
• Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the sterility test room.
• Wait for the fogging to complete.
• As soon as the fogging is complete, quickly transfer the fogger from Sterile Buffer room to Sterility test room and place it similarly.
• Adjust the timer to around 0.7 liter.
• Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the Return Buffer room and close the door.
• As soon as the fogging is complete remove the fogger from the sterility test room and then place the fogger in change room-1.
• Keep the doors between Return buffer & Return A/L and Return A/L & Change room-1 open.
• Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the Microbiology corridor and close the door.
• Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.

• MLT Section:
  • Switch off the AHU of the MLT Section.
  • Enter into the MLT Section as per the entry SOP along with the fogger.
  • Enter the Subculture room and place the fogger in one corner on a SS stool and connect the power cord of the fogger to the mains.
  • Adjust the timer of the fogger to 0.5 liter and switch on the mains.
  • Quickly move out of the room, close the door properly and wait in the change room till the fogging is completed.
  • As soon as the fogging is completed quickly take the fogger out of the subculture room and then fog the MLT room using the same procedure.
  • After the fogging is complete in the MLT room quickly take out the fogger from the

MLT room and place it in one corner of the change room on a SS stool.

• Adjust the motor angle at 45˚C and the timer to 1.0 liter.
• Connect the power cord of the fogger to the mains and switch on the mains and quickly move out of MLT section.
• Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.

• Sterilization area:
  • Switch off the AHU of the Sterilization area.
  • Enter into the Sterilization area as per the entry SOP along with the fogger.
  • Enter the Media decontamination room and place the fogger in one corner on a SS stool and connect the power cord of the fogger to the mains.
  • Adjust the motor angle at 45˚C and the timer to 0.5 liter.
  • Switch on the mains and quickly move out of the room.
  • As soon as the fogging is completed quickly take the fogger out of the Media decontamination room and then fog the Media preparation room, following the same procedure.
  • After the fogging of the Media preparation room, quickly take out the fogger and place in one corner of the sterilization room on a SS stool.
  • Adjust the motor angle at 45˚C and the timer to 1.0 liter.
  • Switch on the mains and quickly move out of the room.
  • Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.
• Dilute and use required volume of Virosil as per Table-I.
• Pour the prepared disinfectant solution in to the tank of fogger prior to entering the area to be fumigated.
• Operate the fogger as per SOP.
• Come out of the room using exit process of the particular area.
• Put the label as ‘Area Under Fogging’. Keep the area under this condition for over night.
• In the next day morning, switch on the AHU. Open the dampers.
• Enter the area as per respective entry procedure. Close all the doors. Remove the Fogging set and exit along with the set.
• Record the fogging details.

 

Table-I. Dilution and Use Volume of Virosil

Name of the Area	Name of Fogging Solution	Volume of fogging Solution Concentrate. (ml)	Volume of WFI/ PW (ml)	Total Volume (ml)
Sterility Testing room	Virosil	150	600	750
Sterile Buffer room	Virosil	150	600	750
Change rooms-2, Change room-3 and Change room-4	Virosil	100	400	500
Return Buffer, Return A/L and Change room-1	Virosil	100	400	500
Sub-culture room	Virosil	100	400	500
MLT room	Virosil	100	400	500
MLT A/L and Change room	Virosil	200	800	1000
Media decontamination room	Virosil	100	400	500
Media preparation room	Virosil	100	400	500
Sterilization area and Washing area	Virosil	200	800	1000

• List of Annexure / Formats
• Fogging Record  – Annexure – I

• References (if any):
  • Fogger Manual
• Reason for Revision:
  • Periodic review
• Abbreviations:
• SOP         : Standard operating procedure
• No.          : Number
• QC          : Quality Control
• QA          : Quality Assurance
• SS           : Stainless Steel
• MLT       : Microbial Limit Test

Annexure – I

Fogging Record

Area : _____________________

Date	Fogging Sol. used	Conc.	Volume	Volume of water	Total Volume	Done By/ Time	Remarks