SOP on Fogging of Microbiology Testing Areas

  • Objective:
    • To lay down the procedure for Fogging of Microbiology Testing Areas
  • Scope:
    • This SOP is applicable for Fogging of Microbiology Testing Areas in Microbiology Laboratory.
  • Responsibility:
    • Chemist or above of Microbiology Laboratory:
    • Head – Microbiology section.
  • Accountability:
    • Head – Quality Control.
  • Procedure:
    • Operation
      • Perform the Fogging of Microbiology once in a week at the end of working hours
      • Perform the fogging on below mentioned areas on different days Sterility testing area.
      • MLT, Subculture room and MLT section corridor and Change room.

3)   Sterilization area

  • Make sure that the area is thoroughly cleaned before fogging.
  • Close all the dampers.
  • Switch off all the AHU.
  • Enter into the area to be fogged as per the SOP along with the fogger (Micromist and Nanotech) filled with the required quantity of dilute disinfectant.
  • Sterility testing area:
    • Switch off the interlocking of the sterility testing area.
  • Enter into change room-4 as per the SOP for the area along with the fogger filled with dilute virosil.
  • Keep the doors between change room-2 & 3 and change room 3 & 4 open.
  • Place the fogger in one corner of change room-4.
  • Adjust the motor angle at 45˚C and the timer to 0.5 liter.
  • Connect the power cord of the fogger to the mains and switch on the fogger and quickly move out of the room into the sterility room and close the door.
  • Wait in the sterility room for the fogging to complete.
  • As soon as the fogging is complete, quickly transfer the fogger from change room-4 to Sterile Buffer room and place similarly.
  • Adjust the timer to around 0.7 liter.
  • Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the sterility test room.
  • Wait for the fogging to complete.
  • As soon as the fogging is complete, quickly transfer the fogger from Sterile Buffer room to Sterility test room and place it similarly.
  • Adjust the timer to around 0.7 liter.
  • Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the Return Buffer room and close the door.
  • As soon as the fogging is complete remove the fogger from the sterility test room and then place the fogger in change room-1.
  • Keep the doors between Return buffer & Return A/L and Return A/L & Change room-1 open.
  • Connect the fogger power cord to the mains and switch on the mains and quickly move out of the room into the Microbiology corridor and close the door.
  • Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.
  • MLT Section:
    • Switch off the AHU of the MLT Section.
    • Enter into the MLT Section as per the entry SOP along with the fogger.
    • Enter the Subculture room and place the fogger in one corner on a SS stool and connect the power cord of the fogger to the mains.
    • Adjust the timer of the fogger to 0.5 liter and switch on the mains.
    • Quickly move out of the room, close the door properly and wait in the change room till the fogging is completed.
    • As soon as the fogging is completed quickly take the fogger out of the subculture room and then fog the MLT room using the same procedure.
    • After the fogging is complete in the MLT room quickly take out the fogger from the

MLT room and place it in one corner of the change room on a SS stool.

  • Adjust the motor angle at 45˚C and the timer to 1.0 liter.
  • Connect the power cord of the fogger to the mains and switch on the mains and quickly move out of MLT section.
  • Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.
  • Sterilization area:
    • Switch off the AHU of the Sterilization area.
    • Enter into the Sterilization area as per the entry SOP along with the fogger.
    • Enter the Media decontamination room and place the fogger in one corner on a SS stool and connect the power cord of the fogger to the mains.
    • Adjust the motor angle at 45˚C and the timer to 0.5 liter.
    • Switch on the mains and quickly move out of the room.
    • As soon as the fogging is completed quickly take the fogger out of the Media decontamination room and then fog the Media preparation room, following the same procedure.
    • After the fogging of the Media preparation room, quickly take out the fogger and place in one corner of the sterilization room on a SS stool.
    • Adjust the motor angle at 45˚C and the timer to 1.0 liter.
    • Switch on the mains and quickly move out of the room.
    • Place a tag “Area Under Fogging”  on the entry door and leave the area undisturbed for overnight.
  • Dilute and use required volume of Virosil as per Table-I.
  • Pour the prepared disinfectant solution in to the tank of fogger prior to entering the area to be fumigated.
  • Operate the fogger as per SOP.
  • Come out of the room using exit process of the particular area.
  • Put the label as ‘Area Under Fogging’. Keep the area under this condition for over night.
  • In the next day morning, switch on the AHU. Open the dampers.
  • Enter the area as per respective entry procedure. Close all the doors. Remove the Fogging set and exit along with the set.
  • Record the fogging details.

 

Table-I. Dilution and Use Volume of Virosil

Name of the Area Name of Fogging

Solution

Volume of fogging

Solution Concentrate. (ml)

Volume of WFI/ PW (ml) Total Volume

(ml)

Sterility Testing room Virosil 150 600 750
Sterile Buffer room Virosil

 

150 600 750
Change rooms-2, Change room-3 and Change room-4 Virosil

 

100 400 500
Return Buffer, Return A/L and Change room-1 Virosil 100 400 500
Sub-culture room Virosil 100 400 500
MLT room Virosil 100 400 500
MLT A/L and Change room Virosil 200 800 1000
Media decontamination room  Virosil 100 400 500
Media preparation room  Virosil 100 400 500
Sterilization area and Washing area Virosil 200 800 1000
  • List of Annexure / Formats
  • Fogging Record  – Annexure – I
  • References (if any):
    • Fogger Manual
  • Reason for Revision:
    • Periodic review
  • Abbreviations:
  • SOP         : Standard operating procedure
  • No.          : Number
  • QC          : Quality Control
  • QA          : Quality Assurance
  • SS           : Stainless Steel
  • MLT       : Microbial Limit Test

Annexure – I

Fogging Record

Area : _____________________

Date Fogging

Sol. used

Conc. Volume Volume of water Total Volume Done

By/ Time

Remarks

 

 

 

 

 

 

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