Saturday , July 4 2020
Home / QA & QC / Microbiology / SOP on Decontamination and Disposal of Used Media

SOP on Decontamination and Disposal of Used Media

  • SOP on Decontamination and Disposal of Used Media
  • Objective

To lay down the procedure for Decontamination and Disposal of Used Media

 Scope

This SOP is applicable for Decontamination and Disposal of Used Media. (Pharmaceutical Company Name).

  • Responsibility
    • Microbiologist or above of Microbiology Laboratory.
    • Head – Microbiology section / Nominee.
  • Accountability
    • Head – Quality Control. / Nominee.
  • Abbreviations and Definitions

SOP         : Standard operating procedure

No.            : Number

QC            : Quality Control

QA            : Quality Assurance

  • Procedure
    • Wear nose mask and disposable hand gloves during scrapping and decontamination activity of used media.
    • For Solidified Media(Media Used for Settle plates exposure, Surface Monitoring

test plates, Microbial limit test plates, Growth promotion test media, Sub-culture slants  etc.):

  • Segregate and arrange the plates showing growth of colonies into biohazard bag.
  • Scrap out the media from the plates having no growth with the help of spatula into another biohazard bag.
  • Place the bags in the Vertical autoclave.
  • For Liquefied Media: (Sterility Tubes/canisters, Positive Control tubes, culture tubes

product enrichment tubes etc.)

  • Place all the test tubes in the test tube stand and arrange them in the Vertical autoclave.
  • Place the Disposable media plates/strips/media kits, used cotton plugs, gloves used

for handling of microbial cultures, lyophilized culture glass pieces, sterility test vent

filters & tubing etc in a biohazard bag and load in the vertical autoclave.

  • Keep one or two deodorizing pearls (if available) in the decontamination load to prevent putrefied smell of the used media.
  • Operate the vertical autoclave as per SOP No
  • After sterilization, collect the media contained in tubes/ bottles and other decontaminated material into biohazard bag.
  • Add 50mL of 20% Virosil solution or 2.5% Savlon to the bag containing decontaminated media and shake the bag for proper dispersion of the added disinfectant solution.
  • Discard the contents of the bag into the washbasin/sink.
  • Open the tap and allow the water to flow while discarding the contents.

 Forms and Records (Annexures)

    • Not applicable
  • Distribution
    • Master copy        –       Quality Assurance
    • Controlled copies –       Quality Assurance, Production, Quality Control, Stores, Engineering   and Human Resource
  • History:
Date Revision Number
Reason for Revision
00   NEW SOP

 

For More Pharma Updates Visit –https://pharmaguidances.com

About Pharmaceutical Guidanace

Mr. Shiv Kumar is the Author and founder of pharmaceutical guidance, he is a pharmaceutical Professional from India having more than 14 years of rich experience in pharmaceutical field. During his career, he work in quality assurance department with multinational company’s i.e Zydus Cadila Ltd, Unichem Laboratories Ltd, Indoco remedies Ltd, Panacea Biotec Ltd, Nectar life Science Ltd. During his experience, he face may regulatory Audit i.e. USFDA, MHRA, ANVISA, MCC, TGA, EU –GMP, WHO –Geneva, ISO 9001-2008 and many ROW Regularities Audit i.e.Uganda,Kenya, Tanzania, Zimbabwe. He is currently leading a regulatory pharmaceutical company as a head Quality. You can join him by Email, Facebook, Google+, Twitter and YouTube

Check Also

Investigating Out-of-Specification

Investigating Out-of-Specification IDENTIFYING AND ASSESSING OOS TEST RESULTS — PHASE I: LABORATORY INVESTIGATION PHASE II:  …