SOP for Operation and Calibration of Total Organic Carbon (TOC)

  • 1.0 OBJECTIVE:To lay down a Standard Operating Procedure for Operation and Calibration of Total Organic Carbon (TOC) Analyzer.
  • 2.0 SCOPE:This procedure shall apply for Operation and Calibration of Total Organic Carbon (TOC) Analyzer.
  • .3.0 RESPONSIBILITY:
    • All concerned personnel shall responsible to follow the procedure mention in this SOP.
    • Concerned Department Heads shall be responsible for compliance of the procedure.

    4.0 ACCOUNTABILITY:

    QC and QA Head shall be accountable that this SOP is implemented as per laid down procedure.

    5.0 ATTACHMENTS:

    Flow chart of Total Organic Carbon (TOC) Investigation & failure result – Attachment – I

    Format for Calibration Recording of Total Organic Carbon (TOC) – Attachment – II

    Checklist for TOC sample failure in the analysis – Attachment – III

    6.0 PROCEDURE:

    General Principle of Total Organic Carbon (TOC) Analyzer

  • TOC Analyzer converts the carbon in the sample to CO2.
  • The NDIR uses electromagnetic radiation or infrared energy to measure this CO2.
  • This measurement is proportional to the carbon in the sample.
  • Carbon dioxide shows a unique adsorption spectrum when IR energy passes through it.

Precautions during analysis of Total Organic Carbon (TOC):

  • For better analysis results, change rinsing water daily and store prepared reagents away from direct sunlight.
  • Use the sucrose and 1,4 Benzoquinone USP reference standard and store the same as per recommend storage condition.
  • Use A-grade glassware for sample collection.
  • In any case don’t rinse the glassware used for Total Organic Carbon (TOC) analysis with any solvent.
  • Glassware required for Total Organic Carbon (TOC) analysis should be cleaned for organic residue, rising with 2N nitric acid then with purified water 3-4 times and dry the TOC sampling container’s for 105°C for 30 min.
  • Preferably such glassware should be maintained separately and to be dedicated for Total Organic Carbon (TOC) analysis
  • At the sample, collection point rinses glassware with sample water 3-4 times
  • The sample should be analyzed immediately after it is received.

7.0 REFERENCES:

ISO 2859-1:1999

8.0 ABBREVIATIONS:

SOP: Standard Operation Procedure

ASI:   Automatic Sample injector

  • NPOC: Nonpurgeable organic carbon
  • TOC: Total Organic Carbon
  • 9.0 DISTRIBBUTION LIST:Quality AssuranceHuman Resource and administrationQuality Control10.0 HISTORY OF REVISION:
    Version No.Effective DateReason for revision

    for Operation and Calibration of Total Organic Carbon (TOC) Analyzer.

 

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