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SOP on Operation, Calibration, Cleaning and Maintenance of LAF

Functioning , Calibration, Cleaning and Maintenance of LAF

Objective:

  • Technique for functioning, Calibration, Cleaning and Maintenance of LAF

Scope:

  • This SOP is relevant for Operation, Calibration, Cleaning and Maintenance of LAF in Microbiology Laboratory.

Responsibility:

  • Chemist or above of Microbiology Laboratory.
  • Head – Microbiology Section.

Accountability:

  • Head – Quality Control.

Procedure:

Make: Clean Room Presentations

LAF

Operation:

  • Plug in the LAF to the Mains Power supply and switch ON the mains.
  • The front panel of the LAF includes the subsequent buttons
  • UNIDIRECTIONAL FLOW
  • FLUORESCENT LIGHT
  • GERMICIDAL  LIGHT
  • Power ‘ ON’ button of UNIDIRECTIONAL FLOW to turn  ON the blower.
  • Power ‘ON’ button of FLUORESCENT LIGHT to turn ON the Light.
  • Power ‘ON’ button of GERMICIDAL LIGHT to turn ON the GERMICIDAL LIGHT.
  • Before Starting the work, Switch on the Blower and GERMICIDAL LIGHT for half-hour. After half-hour transfer off the GERMICIDAL LIGHT and Switch the FLOUORESCENT LIGHT on. Then slowly uplift the the front door up, to open.
  • Monitor the Positive pressure analyzing on Magnehelic gauge. It have to be among 10 and 20 mm of water gauge.
  • Wipe the LAF Work bench with 70% IPA after the work as per requirement.
  • After completion of work , screen the pressure analyzing on Magnehelic gauge.
  • Clean the work station with 70% IPA
  • Close the door.
  • Power ‘OFF’ button of FLUORESCENT LIGHT to turn OFF the Light.
  • Power ‘OFF’ button of UNIDIRECTIONAL FLOW to turn OFF the Blower.
  • Power ON button of GERMICIDAL LIGHT to turn ON the GERMICIDAL LIGHT and keep it for 10 minutes.
  • Then Power OFF button of GERMICIDAL LIGHT to turn OFF the GERMICIDAL LIGHT.
  • Record the LAF usage  in the format given as Annexure No. A01

Calibration:

  • Perform the calibration of Magnehelic gauge once in a year through external agencies

Cleaning and Maintenance:

  • Clean the internal walls, grill and workbench of LAF Cabinet before beginning and after completion of every work with the assist of lint free cloth dipped with disinfectant solution.
  • Clean the outer frame of the LAF the usage of a lint free cloth dipped with disinfectant solution twice (at the start and end of day’s work).
  •  Remove the pre-filter from the LAF for Cleaning purpose.
  • Pre-filter shall be cleaned by maintenance person once in a month with compressed air and soap water.
  • Place the wiped clean Pre-filter at its position. Switch on the LAF unit. Check the positive pressure on Magnehelic gauge. It should be in the middle of 10 and 20 mm of water gauge.
  • If pressure analyzing on Magnehelic gauge is not achieved, put a status label `Under Maintenance’ & ask the Maintenance Department for necessary action.
  •  Change the HEPA filter of the LAF if the reading is above 20 mm of water gauge

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List of Annexure / Formats:

S.

No.

Format Title Annexure

Number

1 LAF Log Book A01

References (if any):

  • Not applicable.

Reason for Revision:

  • Periodic review

Abbreviations:

  • SOP : Standard operating procedure
  • NO: Number
  • QC : Quality Control
  • QA : Quality Assurance
  • LAF : Laminar Air Flow

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