SOP on Operation, Calibration, Cleaning and Maintenance of LAF

Functioning , Calibration, Cleaning and Maintenance of LAF

Objective:

• Technique for functioning, Calibration, Cleaning and Maintenance of LAF

Scope:

• This SOP is relevant for Operation, Calibration, Cleaning and Maintenance of LAF in Microbiology Laboratory.

Responsibility:

• Chemist or above of Microbiology Laboratory.
• Head – Microbiology Section.

Accountability:

• Head – Quality Control.

Procedure:

Make: Clean Room Presentations

Operation:

• Plug in the LAF to the Mains Power supply and switch ON the mains.
• The front panel of the LAF includes the subsequent buttons
• UNIDIRECTIONAL FLOW
• FLUORESCENT LIGHT
• GERMICIDAL  LIGHT
• Power ‘ ON’ button of UNIDIRECTIONAL FLOW to turn  ON the blower.
• Power ‘ON’ button of FLUORESCENT LIGHT to turn ON the Light.
• Power ‘ON’ button of GERMICIDAL LIGHT to turn ON the GERMICIDAL LIGHT.
• Before Starting the work, Switch on the Blower and GERMICIDAL LIGHT for half-hour. After half-hour transfer off the GERMICIDAL LIGHT and Switch the FLOUORESCENT LIGHT on. Then slowly uplift the the front door up, to open.
• Monitor the Positive pressure analyzing on Magnehelic gauge. It have to be among 10 and 20 mm of water gauge.
• Wipe the LAF Work bench with 70% IPA after the work as per requirement.
• After completion of work , screen the pressure analyzing on Magnehelic gauge.
• Clean the work station with 70% IPA
• Close the door.
• Power ‘OFF’ button of FLUORESCENT LIGHT to turn OFF the Light.
• Power ‘OFF’ button of UNIDIRECTIONAL FLOW to turn OFF the Blower.
• Power ON button of GERMICIDAL LIGHT to turn ON the GERMICIDAL LIGHT and keep it for 10 minutes.
• Then Power OFF button of GERMICIDAL LIGHT to turn OFF the GERMICIDAL LIGHT.
• Record the LAF usage  in the format given as Annexure No. A01

Calibration:

• Perform the calibration of Magnehelic gauge once in a year through external agencies

Cleaning and Maintenance:

• Clean the internal walls, grill and workbench of LAF Cabinet before beginning and after completion of every work with the assist of lint free cloth dipped with disinfectant solution.
• Clean the outer frame of the LAF the usage of a lint free cloth dipped with disinfectant solution twice (at the start and end of day’s work).
•  Remove the pre-filter from the LAF for Cleaning purpose.
• Pre-filter shall be cleaned by maintenance person once in a month with compressed air and soap water.
• Place the wiped clean Pre-filter at its position. Switch on the LAF unit. Check the positive pressure on Magnehelic gauge. It should be in the middle of 10 and 20 mm of water gauge.
• If pressure analyzing on Magnehelic gauge is not achieved, put a status label `Under Maintenance’ & ask the Maintenance Department for necessary action.
•  Change the HEPA filter of the LAF if the reading is above 20 mm of water gauge

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List of Annexure / Formats:

S. No.	Format Title	Annexure Number
1	LAF Log Book	A01

References (if any):

• Not applicable.

Reason for Revision:

• Periodic review

Abbreviations:

• SOP : Standard operating procedure
• NO: Number
• QC : Quality Control
• QA : Quality Assurance
• LAF : Laminar Air Flow

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